豚鼠醛化细胞悬液（多种浓度）

 豚鼠醛化细胞悬液（多种浓度）

【产品名称】 ：1%豚鼠醛化细胞悬液

【货号】：FXQ364-01

【产品规格】 ：100ml                            

【储存条件】 ：2～8℃                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

【有效期】 ：三个月 

醛化红细胞特点:
1.醛化红细胞镜检时一般呈两面凸起的园盘状，散在，不聚集成团。
2.红细胞醛化后沉积细胞体 积约增加20%，红细胞固有形态无变化，呈棕色，在有Na+存在时颜色略红。
3.醛化细胞具有较强的抗破坏能力，反复冻融，加热至60℃，蒸馏水中低渗均不破坏，冻干后重新以水悬浮并无明 显变化，pH<3.0时，可自然凝集，但pH达1.8时也不溶血;pH≥10.2可有中度溶血。醛化细胞悬液4℃可存放3个月以上，但冻结后重新融化则凝集成团。 

戊二醛化红细胞制备法:
沉积红细胞用10~20倍0.11MpH7.2 PB洗4~6，再次配成10%悬液，取此悬液 (预先冷至4℃) 缓慢地加入到等量 1%的戊二醛(25%戊二醛用0.11MpH7.2PB稀释 之)中，并继续摇动30~60分钟，然后用蒸馏水洗五次。最后用重蒸馏水 配成20%的悬液，加0.1%NaN3防腐，4℃冰箱保存备用。
丙酮醛一一甲醛化红细胞制备:
(1)沉积红细胞以10~20倍体积 0.11MpH7.2PB洗涤4~6次，然后用该PB配成8%红细胞悬液。
(2)8%红细胞悬液中,缓慢加入等量3%丙酮醛、3%甲醛PB液(取丙酮醛15ml、甲醛8ml、0.11MpH7.2PB60ml,用10%Na-OH调正pH至7.2再加同样PB至100mI)于20℃左右缓慢搅拌17小时，至时用0.11MpH7.2PB洗三次，并用同样PB制成20%红细胞悬液，加入0.1%NaN3防腐，4℃冰箱保存备用。

注意事项：
1.在加醛类前要尽量将红细胞洗净;
2.开始加入甲醛浓度要淡，以后逐渐提高甲醛浓度，否则红细胞容易变形;
3.作适当的摇动，使甲醛与红细胞充分接触。

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 豚鼠醛化细胞悬液（多种浓度）